We developed an assay in which highly purified human TPO is adsorbed to microtiter plate wells; after incubation with the study serum samples, presence of specific antibodies is revealed by a monoclonal europium-labeled antibody against human IgG. The sensitivity of the test is 0.6U/mL, and the reproducibility quite acceptable. We analyzed serum samples from: 188 normal subjects of both sexes, 74 of whom >65 years of age; 54 patients with Graves’ disease (GD) and 13 with Hashimoto thyroiditis (HT). Based […]

Laboratory tests are essential for the diagnosis and follow-up of autoimmune and neoplastic thyroid diseases. The most important test for the etiologic diagnosis of Hashimoto’s thyroiditis, a disease with high prevalence in the population, is the determination of anti-thyroperoxidase antibodies (A-TPO), which is also useful for the diagnosis of Graves’ disease, for predicting risk of hypothyroidism in sub-clinical hypothyroidism and for predicting postpartum thyroiditis in at-risk women. Another very useful test in autoimmune diseases is the determination of anti-TSH receptor […]