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DOI: 10.1590/S0004-27302002000100013
Measurement of steroid hormones has undergone a significant technical evolution so that steroids with greater clinical interest are, nowadays, measured with methods that are simple, fast and prone to automation. Nevertheless, a compromise is made in detriment of accuracy, which is more evident with steroids with lower concentration and more potential interferents, as 17-hydroxyprogesterone (17OHP), dihydrotestosterone (DHT), and others. In such cases, a robust and versatile preparative process is of utmost importance to warrant results with the highest degree of […]
Keywords: 17OHP radioimmunoassay; DHT radioimmunoassay; Hormonal steroids measurement; Preparative HPLC