ABSTRACT Objective: To investigated how miR-195-5p affects oxidative stress and modulates aerobic metabolism. Materials and methods: MiR-195-5p plus GLS2 mRNA was identified by conducting real-time quantitative polymerase chain reaction. Western blotting was conducted to determine GLS2 protein expression. Corresponding kits were used to determine the concentrations of glutamate, reduced glutathione, oxidized glutathione, a-ketoglutarate, and adenosine triphosphate. The cell counting Kit-8 assay was performed to determine viability. Flow cytometry assay was performed to measure the reactive oxygen species content. Finally, a […]

MicroRNAs (miRNAs) represent a novel class of endogenous ~22-nucleotide RNAs that negatively regulate gene expression by inhibiting translation of target RNAs. Discovered just over a decade ago in Caenorhabditis elegans, miRNAs are now recognized as one of the major regulatory gene families in plants and animals. In the human genome, 462 miRNA genes have been discovered and the estimated number of miRNAs is as high as 1000. Bioinformatics analysis indicated that a unique miRNA acts on several mRNA, influencing multiple […]