An excessive level of these species may be harmful as it may overcome the antioxidant capacity. Most evidences for ROS and NO formation in muscle cell are indirect whereas direct evidences are scarce. The present review suggests as an alternative method either fluorescent DCFH, DAF-2 or fluorescamine toward H2O2, NO and O2·- measurements, respectively in muscle cell. In adition, it was concluded that: 1) the “indirect” measurements of ROS and NO in blood sample do not reflect oxidative attack occured […]

The main cause for mortality and the great morbidity in diabetes (DM) is atherosclerosis. A hypothesis for the initial lesion of atherosclerosis is endothelial dysfunction by recognizing that the endothelium plays a fundamental role in the maintenance of blood fluidity, vessel wall tone and permeability, the endothelium-derived nitric oxide (e-NO) being the main protector against atherosclerotic disease by inhibiting key pathways involved in atherogenesis. Endothelial dysfunction identified as an early marker of cardiovascular disease (CVD) could predict coronary artery disease […]